MS PDA-UniQ : Yu-Cheng Huang, Bioinformatics Lab, CSIE, NTU

Minimum Set Primers and Unique Probes Design Algorithms for

Differential Detection of Symptom-Related Pathogens

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Introduction
Methodology
Computational Results
Bio-Experiment
  9 Plant Virus (Table 3)
 

Primers & Probes   (Table 4, 5)
  Experimental Result
Conclusion
Reference

Table 3. The 9 plant viruses from 4 genus.

Table 4. The minimum set primers for the 9 plant viruses.

Table 5. Probes and primers for each plant virus. (Probe length = 50)

a The melting temperature of the unique probe.

b The position of the start base.

c The forward primer used.

d The reverse primer used.

e The length of sequence amplified by the specific primers, in b.p.